The effects of Syzygium aromaticum (Clove) Leaf extracts on Rats Sperm Function

• Abdulkarem Ilfergane

  Department of Zoology, Faculty of Science, University of Benghazi

  Author

• Eman Ali Farag Hamouda

  Department of Zoology, Faculty of Humanities and Applied Sciences, University of Benghazi, Tocra, Libya

  Author

Syzygium aromaticum (Clove) is a small evergreen tree of the myrtle family known for its unopened reddish-brown flower bud with strong aromatic flavor that are used for culinary purpose and are believed to have aphrodisiac properties, including those related to fertility and infertility diseases. The flower buds of Syzygium aromaticum (clove) have been used in indigenous medicines for the treatment of male sexual disorders in Indian subcontinent. Objective to evaluate the effect of Syzygium aromaticum flower bud on male reproduction, using rats as animal model. The aim of this study was to design a dose-response assessment to assess the effects of aqueous leaf extract of Syzygium aromaticum. Healthy adult male rats were treated with 1, 10 and 100 mg/kg/day of Syzygium aromaticum leaf extract by treated for 45 days. Then assess the weight, Sperm morphology, DNA fragmentation, sperm viability, sperm morphology and sperm motility of the reproductive organs. Results: There was no effect on body weight, There is a positive effect in rats treated with Syzygium aromaticum extract at low doses at 1mg/kg/day, sharp and significant decrease in all vital reproductive functions in rats treated with Syzygium aromaticum extract at high concentration at 10 and 100 mg/kg/day. The explanation for the increase in sperm functionality might have been induced by the presence of components within the plant of Syzygium aromaticum causing the effect on spermatozoa enabling them to reach optimal motility. Thereby enhancing the negative effects observed within this study. This study indicated that the traditional use of this herbs has harmful effects on male reproductive health at high doses. This study underscores the potential of Clove leaf fractions as natural aphrodisiacs, warranting further investigation for clinical applications. Conclusion: Treatment with Syzygium aromaticum flower bud causes dose‑dependent biphasic effect on male reproductive indices in Rats; lower dose of Syzygium appears stimulatory, while the higher doses have adverse effect on male reproduction. The results suggest that the lower dose of Syzygium may have androgenic effect, but further studies are needed to support this contention.

1. Eman Ali Farag Hamouda، Department of Zoology, Faculty of Humanities and Applied Sciences, University of Benghazi, Tocra, Libya

   Department of Zoology, Faculty of Humanities and Applied Sciences, University of Benghazi, Tocra, Libya

Agbaje EO, Adeneye AA, Daramola AO (2009). Biochemical and toxicological studies of aqueous extract of Syzygium aromaticum (Myrtaceae) in rodents. Afr J Trad CAM, 6(3): 241-254. DOI: https://doi.org/10.4314/ajtcam.v6i3.57162

Alfikri F. N., Pujiarti R., Yield (2020). Quality, and Antioxidant activity of clove (Syzygium aromaticum L. Bud oil at the different Phenological Stages in Young and Mature Trees, Toxicol Appl Pharmacol, 20 (7). DOI: https://doi.org/10.1155/2020/9701701

Auerbach SS, Shah RR, Mav D, Smith CS, Walker NJ, Vallant MK, Boorman GA, Irwin RD (2010). Predicting the hepatocarcinogenic potential of alkenylbenzene flavouring agents using toxicogenomics and machine learning. Toxicol Appl Pharmacol, 243: 300-314. DOI: https://doi.org/10.1016/j.taap.2009.11.021

Batiha G. E-S. ,Alkazmi L. M (2020). Syzygium aromaticum L. (Myrtaceae): Traditional uses, Bioactivechemical constituents, pharmacological and toxicological activities: 12 (5).

Biernasiuk, A., Baj, T., & Malm, A. (2022). Clove essential oil and its main constituent, eugenol, as potential natural antifungals against Candida spp. alone or in combination with other antimycotics due to synergistic interactions. Molecules, 28(1), 215. DOI: https://doi.org/10.3390/molecules28010215

Brunetti P, Lo Faro AF, Tini A, Busardò FP, Carlier J (2020). Pharmacology of Herbal Sexual Enhancers: A Review of Psychiatric and Neurological Adverse Effects. Pharmaceuticals. Oct 14; 13(10):309. DOI: https://doi.org/10.3390/ph13100309

Chandra Manivannan, A., Malaisamy, A., Eswaran, M., Meyyazhagan, A., Arumugam, V. A., Rengasamy, K. R., & Liu, W. C. (2022). Evaluation of clove phytochemicals as potential antiviral drug candidates targeting SARS-CoV-2 Main protease: computational docking, molecular dynamics simulation, and pharmacokinetic profiling. Molecular Biosciences, 9, 918101. DOI: https://doi.org/10.3389/fmolb.2022.918101

Dos Santos PE, Egito LC, Medeiros SR, Agnez-Lima LF (2008). Genotoxicity induced by Eugenia caryophyllata infusion. J Toxicol Environ Health. 71:439-44. DOI: https://doi.org/10.1080/15287390701839232

Dua Anita, Singh Avtar, Mahajan Ritu (2015). Antioxidants of clove (syzygium aromaticum) prevent metal induced oxidative damage of biomolecules: Int. Res j. Pharm, 6 (4). DOI: https://doi.org/10.7897/2230-8407.06460

El-Saber Batiha G, Alkazmi LM, Wasef LG, Beshbishy AM, Nadwa EH, Rashwan EK. Syzygium aromaticum (2020). Traditional Uses, Bioactive Chemical Constituents, Pharmacological and Toxicological Activities. Biomolecules. Jan 30; 10(2):202. DOI: https://doi.org/10.3390/biom10020202

Han, X., & Parker, T. L. (2017). Anti-inflammatory activity of clove (Eugenia caryophyllata) essential oil in human dermal fibroblasts. Pharmaceutical Biology, 55(1), 1619- 1622. DOI: https://doi.org/10.1080/13880209.2017.1314513

Haro-González, J. N., Castillo-Herrera, G. A., Martínez-Velázquez, M., & Espinosa-Andrews, H. (2021). Clove essential oil (Syzygium aromaticum L. Myrtaceae): Extraction, chemical composition, food applications, and essential bioactivity for human health. Molecules, 26(21), 6387. DOI: https://doi.org/10.3390/molecules26216387

Hatami, T., Johner, J. C., Zabot, G. L., & Meireles, M. A. A. (2019). Supercritical fluid extraction assisted by cold pressing from clove buds: Extraction performance, volatile oil composition, and economic evaluation. The Journal of Supercritical Fluids, 144, 39-47. DOI: https://doi.org/10.1016/j.supflu.2018.10.003

Kaur K, kaushal S, (2018). Phytochemistry and Pharmacological aspects of Syzygium Aromaticum: A review, 8(1): 398- 406.

Mishra RK, Singh SK (2008). Safety assessment of Syzygium aromaticum flower bud (clove) extract with respect to testicular function in mice. Food Chem Toxicol, 46: 3333-3338. DOI: https://doi.org/10.1016/j.fct.2008.08.006

Mostafa, A. A. F., Yassin, M. T., Al–Askar, A. A., & Al-Otibi, F. O. (2023). Phytochemical analysis, antiproliferative and antifungal activities of different Syzygium aromaticum solvent extracts. Journal of King Saud University-Science, 35(1), 102362. DOI: https://doi.org/10.1016/j.jksus.2022.102362

Pulikottil, S. J., & Nath, S. (2015). Potential of clove of Syzygium aromaticum in development of a therapeutic agent for periodontal disease, a review. South African Dental Journal, 70(3), 108-115.

Radünz, M., da Trindade, M. L. M., Camargo, T. M., Radünz, A. L., Borges, C. D., Gandra, E. A., & Helbig, E. (2019). Antimicrobial and antioxidant activity of unencapsulated and encapsulated clove (Syzygium aromaticum, L.) essential oil. Food Chemistry, 276, 180-186. DOI: https://doi.org/10.1016/j.foodchem.2018.09.173

Sarker, J., & Islam, M. N. (2022). Comparative summary of the ethnomedicinal use, phytochemical constituents, and pharmacological properties of Syzygium aromaticum and Ocimum sanctum. Pharmacother. Pharmascience Discov, 1, 82-100.

Sarker, J., & Islam, M. N. (2022). Comparative summary of the ethnomedicinal use, phytochemical constituents, and pharmacological properties of Syzygium aromaticum and Ocimum sanctum. Pharmacother. Pharmascience Discov, 1, 82-100. DOI: https://doi.org/10.66944/PtherPsciD/PPD21111

Somrani, M., Debbabi, H., & Palop, A. (2022). Antibacterial and antibiofilm activity of essential oil of clove against Listeria monocytogenes and Salmonella enteritidis. Food Science and Technology International, 28(4), 331-339. DOI: https://doi.org/10.1177/10820132211013273

Srivastava KC, Malhotra N (1991). Acetyl eugenol, a component of oil of cloves (S. aromaticum) inhibits aggregation and alters arachidonic acid metabolism in human blood platelets. Prostaglandins Leukot Essent Fatty Acids. Pharmaceutical Biology, 42:73-78. DOI: https://doi.org/10.1016/0952-3278(91)90070-L

Tajuddin, Ahmad S, Latif A, Qasmi IA (2003). Aphrodisiac activity of 50% ethanolic extracts of Myristica fragrans Houtt. (Nutmeg) and Syzygium aromaticum (L.) Merr. & Perry. (Clove) in male mice: a comparative study. BMC complement Altern Med, 3:6. DOI: https://doi.org/10.1186/1472-6882-3-6

Vijayasteltar, L., Nair, G. G., Maliakel, B., Kuttan, R., & Krishnakumar, I. M. (2016). Safety assessment of a standardized polyphenolic extract of clove buds: Subchronic toxicity and mutagenicity studies. Toxicology Reports, 3, 439-449. DOI: https://doi.org/10.1016/j.toxrep.2016.04.001

Zari, A. T., Zari, T. A., & Hakeem, K. R. (2021). Anticancer properties of eugenol: A review. Molecules, 26(23), 7407. DOI: https://doi.org/10.3390/molecules26237407

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