Prevalence and Risk Factors of Gastrointestinal Parasites in Horses in Aljabal Alakhdar region, Libya.
- Authors
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Somia A. Alsanousi
Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, Omar Al-Mukhtar University, LibyaAuthor -
Ahmed Bulabiad
Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, Omar Al-Mukhtar University, LibyaAuthor -
Najwa A. I. Abdulsalam
Author -
Mohamed Bengheshir
Department of Parasitology, Faculty of Medicine, University of Benghazi, LibyaAuthor
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- Keywords:
- Prevalence, Gastrointestinal parasites, Cyathostomins, Faecal egg count, Libya.
- Abstract
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Libyan equines play an important role in traditional culture, and like all grazing animals, they often suffer from gastrointestinal parasite infections, especially in breeding facilities. These infections can lead to serious health problems and financial losses. In eastern Libya, data on these parasitic populations are limited due to limited research; therefore, this study aimed to identify the gastrointestinal parasite in horses in the region. Fresh faecal samples were collected from 110 horses from different areas of Aljabal Alakhdar to determine the prevalence, associated risk factors, and to identify the genera of equine gastrointestinal parasites. The flotation technique and the concentration-modified McMaster method were used to perform faecal egg count analysis (FEC) to estimate the number of faecal eggs per gram (EPG). The overall prevalence of GI parasites in horses was 59.09%, and the most dominant type was Cyathostomins species, found in 58.18% of the cases. Whereas Parascaris species was present in 3.64%, and Oxyuris equi was present in 2.73% of the infected horses. Both Eimeria species and Gasterophilus species were found in 0.91% of the examined cases, indicating they were relatively uncommon. However, no significant associations were found between regions, seasons, deworming practices, ages, and breeds of infected animals. In conclusion, gastrointestinal parasite infections present a considerable health risk to equines in the study area. Efforts should focus on improving housing conditions and the feeding system, increasing awareness of welfare, and implementing seasonal anthelminthics management.
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- References
-
Abd-Elrahman, S., Dyab, A., Mohamed, S., & Abd-Elrady, L. (2021). Prevalence of gastrointestinal parasites in horses in Luxor Egypt. Assiut Veterinary Medical Journal, 67. doi:https://doi.org/10.21608/avmj.2021.95333.1015.
Alashger, E. M., Elsheikh, K. M. K., Alfatory, N. Y., & Eljamay, S. M. (2025). Molecular Variability Among Fusarium Oxysporum Isolates Infecting Plants in Different Agricultural Environments in Libya. Derna Academy Journal for Applied Sciences, 3(2), 206-214.
Agela, F. M., Bofares, Z. M., Elzuni, A. F., Ali, B. A. B., Emsallam, S. M., Shretih, G. M., ... & Zabih, N. W. B. (2025). Assessment of Parasitic Contamination in Imported Fresh Vegetables and Comparative Analysis with Locally Grown Vegetables in Derna, Libya. Derna Academy Journal for Applied Sciences, 4(1), 90-96.
Amer, M. M., Desouky, A. Y., Helmy, N. M., Abdou, A. M., & Sorour, S. S. (2022). Identifying 3(rd) larval stages of common strongylid and non-strongylid nematodes (class: Nematoda) infecting Egyptian equines based on morphometric analysis. BMC Vet Res, 18(1), 432. doi:https://doi.org/10.1186/s12917-022-03526-8.
Assir, A. M. (2025). Prevalence of Helicobacter Pylori Among Outpatients at Al-Khadra Hospital, Tripoli: A Cross-Sectional Study. Derna Academy Journal for Applied Sciences, 4(2), 101-105.
Alsanousi, S. A., Atiyahullah, T. M. O., Meriz, O. M., Adam, A. F. H., & Abdulsalam, N. A. I. (2025). The First Record of Ovine Eimeria Species (Apicomplexa) and their Prevalence in Aljabal Alakhdar, Libya. International Journal of Veterinary Science, 14(4), 661-667. doi: https://doi.org/10.47278/journal.ijvs/2025.021
Alsanousi, S. A., & Mohammed, Z. M. A. (2024). First Record of Muellerius Capillaris (Protostrongylidae) and Their Pathologic Changes in Libya. African Journal of Biological Sciences, 6(8), 1588-1599. doi:https://doi.org/10.33472/AFJBS.6.8.2024.1588-1599
Boelow, H., Krücken, J., & von Samson-Himmelstjerna, G. (2023). Epidemiological study on factors influencing the occurrence of helminth eggs in horses in Germany based on sent-in diagnostic samples. Parasitology Research, 122(3), 749-767. doi:https://doi.org/10.1007/s00436-022-07765-4
Ceylan, O., DİK, B., Ceylan, C., Semassel, A., DerİNbay EkİCİ, Ö., & SÖNmez, G. (2020). Gastrointestinal helminths detected in wild horses in Konya Province, Turkey. Turkish Journal of Veterinary and Animal Sciences, 44(3), 662-667. doi:https://doi.org/10.3906/vet-1910-83
Devkota, R. P., Subedi, J. R., & Wagley, K. (2021). Prevalence of gastrointestinal parasites in equines of Mustang District, Nepal. Biodiversitas Journal of Biological Diversity, 22(9). doi:https://doi.org/10.13057/biodiv/d220943
Elghryani, N., McOwan, T., Mincher, C., Duggan, V., & de Waal, T. (2023). Estimating the Prevalence and Factors Affecting the Shedding of Helminth Eggs in Irish Equine Populations. Animals (Basel), 13(4). doi:https://doi.org/10.3390/ani13040581
Elmajdoub, L. O., Mosaab, O., Alsaghir, O. A., & Shimaa, S. S. (2022). Investigation and Prevalence of Gastrointestinal Parasites of Equestrian Clubs Horses in Misurata, Libya. European Journal of Biology and Biotechnology, 3(6), 5-9. doi:https://doi.org/10.24018/ejbio.2022.3.6.414
Emeto, U. E., Okolo, C. C., & Nweze, N. E. (2022). Strongyliasis Occurs in Epidemic Proportion amongst other Nematodiasis and Cestodiasis of Horses (Equus caballus) in Obollo-Afor Southeastern Nigeria. Veterinary Sciences: Research and Reviews, 8(1). doi:https://doi.org/10.17582/journal.vsrr/2022.8.1.15.22
Fleming, S. A., Nielsen, M. K., & Ballweber, L. R. (2020). • Chapter 49 - Parasite Control Programs. In B. P. Smith, D. C. Van Metre, & N. Pusterla (Eds.), Large Animal Internal Medicine (Sixth Edition) (pp. 1669-1693.e1665). St. Louis (MO): Mosby.https://doi.org/10.1016/B978-0-323-55445-9.00049-5.
(ESCCAP)., E. S. C. C. A. P. (2019). A guide to the treatment and control of equine gastrointestinal parasite infections. ESCCAP Guideline 08. 2nd ed. Malvern, United Kingdom.: ESCCAP.
Foreyt, W. J. (2001). veterinary parasitology: reference manual 5th ed.: Wiley Blackwell.
Hedberg-Alm, Y., Penell, J., Riihimäki, M., Osterman-Lind, E., Nielsen, M. K., & Tydén, E. (2020). Parasite Occurrence and Parasite Management in Swedish Horses Presenting with Gastrointestinal Disease-A Case-Control Study. Animals (Basel), 10(4). doi:https://doi.org/10.3390/ani10040638
Hildebrand, W., Zielińska, P., Hildebrand, J., & Zaleśny, G. (2023). Helminth infection in horses - a cross-sectional study from stables in Lower Silesia (Poland). Pol J Vet Sci, 26(3), 401-407. doi:https://doi.org/10.24425/pjvs.2023.145046
Kornaś, S., Cabaret, J., Skalska, M., & Nowosad, B. (2010). Horse infection with intestinal helminths in relation to age, sex, access to grass and farm system. Veterinary Parasitology, 174(3), 285-291. doi:https://doi.org/10.1016/j.vetpar.2010.09.007
Kukurić, T., Erdeljan, M., Matthews, J. B., Lightbody, K. L., Austin, C. J., Peczak, N., . . . Simin, S. (2025). A Prevalence Study on Anoplocephala spp. in Serbian Horses: Navigating Diagnostic Challenges and Understanding Infection Risks. 15(14), 2094. doi:https://doi.org/10.3390/ani15142094
Kumar, P., Vohra, S., Kumar, R., & Ramkaran. (2024). Prevalence of gastrointestinal parasites in Equines of Haryana in relation to their feeding history. International Journal of Advanced Biochemistry Research, 8(6S), 779-781. doi:https://doi.org/10.33545/26174693.2024.v8.i6Sj.1413
Kuzmina, T. A., Dzeverin, I., & Kharchenko, V. A. (2016). Strongylids in domestic horses: Influence of horse age, breed and deworming programs on the strongyle parasite community. Vet Parasitol, 227, 56-63. doi:https://doi.org/10.1016/j.vetpar.2016.07.024
Larsen, M. M., Lendal, S., Chriél, M., Olsen, S. N., & Bjørn, H. (2002). Risk Factors for High Endoparasitic Burden and the Efficiency of a Single Anthelmintic Treatment of Danish Horses. Acta Veterinaria Scandinavica, 43(2), 99. doi:https://doi.org/10.1186/1751-0147-43-99
Libya, E. o. (2020). El Maiz: Libya’s equestrian tradition. Retrieved from https://www.embassyoflibyadc.org/news/el-maiz-libyas-equestrian-tradition
Lind, E. O., Rautalinko, E., Uggla, A., Waller, P. J., Morrison, D. A., & Höglund, J. (2007). Parasite control practices on Swedish horse farms. Acta Veterinaria Scandinavica, 49(1), 25. doi:https://doi.org/10.1186/1751-0147-43-99
Lloyd, S. (2009). Effects of previous control programmes on the proportion of horses shedding small numbers of strongyle-type eggs. Vet Rec, 164(4), 108-111. doi:https://doi.org/10.1136/vr.164.4.108
Love, S., Murphy, D., & Mellor, D. (1999). Pathogenicity of cyathostome infection. Vet Parasitol, 85(2-3), 113-121; discussion 121-112, 215-125. doi:https://doi.org/10.1016/s0304-4017(99)00092-8
Lu, Y., Ru, P., Qin, S., Zhang, Y., Fu, E., Cai, M., . . . Zhang, Y. (2025). Epidemiological Patterns of Gastrointestinal Parasitic Infections in Equine Populations from Urumqi and Ili, Xinjiang, China. Veterinary Sciences, 12(7), 644. doi:https://doi.org/10.3390/vetsci12070644
Malek, I. A., Baharudin, F., Peng, T. L., & Sabri, J. (2025). Development and Survivability of The Free-Living Stage Larvae of Equine Strongyles in Different Environments and Soil Types. Trop Life Sci Res, 36(3), 101-120. doi:https://doi.org/10.21315/tlsr2025.36.3.5
Mangassa, B., & Tafese, W. (2016). Prevalence of Strongyle Infection and Associated Risk Factors in Horse and Donkeys in and Around Batu Town, Eastshoa, Oromia Regional State, Ethiopia. Food Science and Quality Management, 54(2016), 66-71. doi:https://www.iiste.org/Journals/index.php/FSQM/article/view/32483
Marshall, E. (2001). Constructing the self and the other in Cyrenaica. In R. Laurence & J. Berry (Eds.), cultural identity on the roman empire (pp. 49-63): Psychology Press.
Matthews, J. B., & Mair, T. S. (2025). Sustainable control of cyathostomin infections in practice. Equine Veterinary Education, 37(5). doi:https://doi.org/10.1111/eve.14182
Matthews, J. B., Peczak, N., & Lightbody, K. L. (2023). The Use of Innovative Diagnostics to Inform Sustainable Control of Equine Helminth Infections. 12(10), 1233. doi:https://doi.org/10.3390/pathogens12101233
Merlin, A., Larcher, N., & Vallé-Casuso, J. C. (2024). The first report of triple anthelmintic resistance on a French Thoroughbred stud farm. Int J Parasitol Drugs Drug Resist, 24, 100528. doi:https://doi.org/10.1016/j.ijpddr.2024.100528
Mohammed, A., Tukur, S. M., Kayeri, B. K., Sani, M., Mustapha, A. H., Midala, C. A., . . . Ijaw, B. B. (2025). Prevalence of gastrointestinal parasites of the horse in Maiduguri metropolis, Borno State. Sokoto Journal of Veterinary Sciences, 23(1), 38-45. doi:https://doi.org/10.4314/sokjvs.v23i1.4
Mulaw Berihun, A., Bizu, F., Maru, M., & Kassaw, S. (2024). Prevalence, Associated Risk Factors, and Identification of the Genera of Equine Strongyles in Horses and Donkeys in and Around Bishoftu, Ethiopia. Vet Med Int, 2024, 3224113. doi:https://doi.org/10.1155/vmi/3224113
Noble, G. K. (2023). Horse Husbandry–Nutrition, Management and Welfare. 13(1), 169. doi:https://doi.org/10.3390/ani13010169
Osterman-Lind, E., Holmberg, M., & Grandi, G. (2023). Selective Anthelmintic Treatment in Horses in Sweden Based on Coprological Analyses: Ten-Year Results. Animals (Basel), 13(17). doi:https://doi.org/10.3390/ani13172741
Panova, O., Baranova, M., Kurnosova, O., & Khrustalev, A. (2023). Horse gastrointestinal parasites in the Moscow region. BIO Web of Conferences, 71. doi:https://doi.org/10.1051/bioconf/20237101064
Phetkarl, T., Fungwithaya, P., Lewchalermvong, K., & Sontigun, N. (2024). Prevalence of gastrointestinal and blood parasites in horses of Nakhon Si Thammarat province, Thailand. Vet World, 17(11), 2460-2468. doi:https://doi.org/10.14202/vetworld.2024.2460-2468
Pinilla, J. C., Pinilla Da Silva, A. I., Ardila Méndez, M. S., & Vega Navarro, M. A. (2025). Prevalence of gastrointestinal parasites in slaughterhouse horses and donkeys from the North and Northeast of Colombia. Innovaciencia, 13(1). doi:https://doi.org/10.15649/2346075x.4917
Relf, V. E., Morgan, E. R., Hodgkinson, J. E., & Matthews, J. B. (2013). Helminth egg excretion with regard to age, gender and management practices on UK Thoroughbred studs. Parasitology, 140(5), 641-652. doi:https://doi.org/10.1017/S0031182012001941
Roepstorff, A., & Nansen, P. (1998). Epidemiology, diagnosis and control of helminth parasites of swine (1st ed ed. Vol. 3). Rome, Italy Fao Rome.https://openknowledge.fao.org/handle/20.500.14283/x0520e
Romero, C., Heredia, R., Miranda, L., & Arredondo, M. (2020). Prevalence of Gastrointestinal Parasites in Horses of Central Mexico. Open Journal of Veterinary Medicine, 10(08), 117-125. doi:https://doi.org/10.4236/ojvm.2020.108010
Salem, S. E., Abd El-Ghany, A. M., Hamad, M. H., Abdelaal, A. M., Elsheikh, H. A., Hamid, A. A., . . . Ras, R. (2021). Prevalence of gastrointestinal nematodes, parasite control practices and anthelmintic resistance patterns in a working horse population in Egypt. Equine Vet J, 53(2), 339-348. doi:https://doi.org/10.1111/evj.13325
Sallé, G., & Cabaret, J. (2015). A survey on parasite management by equine veterinarians highlights the need for a regulation change. Veterinary record open, 2(2), 1-8. doi:https://10.1136/vetreco-2014-000104
Santos, D. W., Madeira de Carvalho, L. M., & Molento, M. B. (2018). Identification of third stage larval types of cyathostomins of equids: An improved perspective. Vet Parasitol, 260, 49-52. doi:https://doi.org/10.1016/j.vetpar.2018.08.007
Scala, A., Tamponi, C., Sanna, G., Predieri, G., Dessi, G., Sedda, G., . . . Varcasia, A. (2020). Gastrointestinal Strongyles Egg Excretion in Relation to Age, Gender, and Management of Horses in Italy. Animals (Basel), 10(12). doi:https://doi.org/10.3390/ani10122283
Scala, A., Tamponi, C., Sanna, G., Predieri, G., Meloni, L., Knoll, S., . . . Varcasia, A. (2021). Parascaris spp. eggs in horses of Italy: a large-scale epidemiological analysis of the egg excretion and conditioning factors. Parasit Vectors, 14(1), 246. doi:https://doi.org/10.1186/s13071-021-04747-w
Slivinska, K., Gawor, J., & Jaworski, Z. (2009). Gastro-intestinal parasites in yearlings of wild Polish primitive horses from the Popielno Forest Reserve, Poland. Helminthologia, 46(1), 9-13. doi:https://doi.org/10.2478/s11687-009-0002-2
Tahir, U. B., Sajid, M. S., Khan, M., Saqib, M., Siddique, R. M., & Qudoos, A. (2016). Passive surveillance and risk analysis of gastrointestinal parasitism in equine population of Faisalabad Metropolitan, Pakistan. Journal of Animal Plant Sciences, 26, 395-400. doi:https://api.semanticscholar.org/CorpusID:40198086
Taylor, M. A., Coop, R. L., & Wall, R. (2016). Veterinary parasitology: John Wiley & Sons.
Villa-Mancera, A., Aldeco-Pérez, M., Molina-Mendoza, P., Hernández-Guzmán, K., Figueroa-Castillo, J. A., & Reynoso-Palomar, A. (2021). Prevalence and risk factors of gastrointestinal nematode infestation of horses, donkeys and mules in tropical, dry and temperate regions in Mexico. Parasitology International, 81, 102265. doi:https://doi.org/10.1016/j.parint.2020.102265
Von Samson-Himmelstjerna, G., Traversa, D., Demeler, J., Rohn, K., Milillo, P., Schurmann, S., . . . Boeckh, A. (2009). Effects of worm control practices examined by a combined faecal egg count and questionnaire survey on horse farms in Germany, Italy and the UK. Parasit Vectors, 2 Suppl 2(Suppl 2), S3. doi:https://doi.org/10.1186/1756-3305-2-S2-S3
Zajac, A. M., & Conboy, G. A. (2012). Veterinary Clinical Parasitology 8th ed.: A John Wiley & Sons, Inc., Publication.https://www.scribd.com/document/526017532/Veterinary-clinical-parasitology-Zajac-A
Zajac, A. M., Conboy, G. A., Little, S. E., & Reichard, M. V. (2021). Veterinary Clinical Parasitology 9th ed. : Wiley-Blackwell.https://vetbooks.ir/veterinary-clinical-parasitology-9th-edition/
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Copyright (c) 2026 Somia A. Alsanousi , Ahmed Bulabiad , Najwa A. I. Abdulsalam , Mohamed Bengheshir (Author)

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