MICROBIOLOGICAL ASSESSMENT OF COMPUTER KEYBOARDS IN EDUCATIONAL LABORATORIES: A STUDY FROM AL-HIGHER INSTITUTE OF SCIENCE AND TECHNOLOGY / TAMAZAWA – AL-SHATI
- Authors
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Adam Abubaker Mussa Jummh Abubaker Mussa Jummh
Libyan Authority for Scientific ResearchAuthor
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- Keywords:
- Fomites; Bacterial Contamination; Educational Laboratories; Libya.
- Abstract
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Computer keyboards are frequently touched surfaces that may act as fomites for the transmission of microorganisms, particularly in educational laboratory environments. This study aimed to assess the level of bacterial contamination on computer keyboards used in university laboratories in the Tamazawa–Alshati region, Libya. A total of 30 computer keyboards from two university laboratories were sampled using sterile swabs. Samples were cultured on standard bacteriological media, and bacterial isolates were identified using conventional microbiological methods. Bacterial contamination was estimated semi-quantitatively based on colony-forming unit (CFU) counts and categorized into four contamination levels. All sampled keyboards showed bacterial growth, indicating a contamination rate of 100%. A total of 42 bacterial isolates were recovered, with coagulase-negative staphylococci (CoNS) being the most prevalent organisms. Other isolated bacteria included Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Most keyboards demonstrated moderate to heavy levels of contamination. Conclusion, computer keyboards in university laboratories may serve as reservoirs for opportunistic and potentially pathogenic bacteria. The implementation of regular disinfection protocols, improved hand hygiene practices, and increased user awareness is recommended to reduce microbial contamination and potential health risks.
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- References
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AlGhazali, M. A., & Eljamay, S. M. (2025). THE ROLE OF TEMPERATURE IN MICROBIAL GROWTH: A DISCUSSION AND META-ANALYSIS. Derna Academy Journal for Applied Sciences, 4(1), 34-44. DOI: https://doi.org/10.71147/wdxm5r37
Bhoonderowa, A., Gookool, S. and Biranjia-Hurdoyal, S.D. (2014). The importance of mobile phones in the possible transmission of bacterial infections in the community. Journal of Community Health, 39(5), pp. 965–967. DOI: https://doi.org/10.1007/s10900-014-9838-6
Boyce, J.M. (2016). Modern technologies for improving cleaning and disinfection of environmental surfaces in hospitals. American Journal of Infection Control, 44(5 Suppl), pp. e38–e46. DOI: https://doi.org/10.1186/s13756-016-0111-x
Brady, R.R.W., Hunt, A.C., Visvanathan, A., Rodrigues, M.A., Graham, C., Rae, C. and Kalima, P. (2011). Mobile phone technology and hospitalised patients: A cross-contamination risk? Journal of Hospital Infection, 77(4), pp. 362–365.
Cheesbrough, M. (2006). District Laboratory Practice in Tropical Countries. 2nd edn. Cambridge: Cambridge University Press. DOI: https://doi.org/10.1017/CBO9780511543470
Elkholy, M.T. and Ewees, I.E. (2010). Mobile phones are reservoirs of nosocomial pathogens. African Journal of Biotechnology, 9(3), pp. 346–351.
Elzouki, E. M., Eljamay, S. M., & Alshilwi, Y. A. (2024). Isolation and Identification of Escherichia Coli in Uropathogenesis. Derna Academy Journal for Applied Sciences, 2(2), 103-110. DOI: https://doi.org/10.71147/aanfpk05
Kampf, G., Todt, D., Pfaender, S. and Steinmann, E. (2017). Persistence of coronaviruses on inanimate surfaces and their inactivation with biocidal agents. Journal of Hospital Infection, 104(3), pp. 246–251. DOI: https://doi.org/10.1016/j.jhin.2020.01.022
Kramer, A., Schwebke, I. and Kampf, G. (2006). How long do nosocomial pathogens persist on inanimate surfaces? BMC Infectious Diseases, 6, p. 130. https://doi.org/10.1186/1471-2334-6-130 DOI: https://doi.org/10.1186/1471-2334-6-130
Neely, A.N. and Maley, M.P. (2000) Survival of enterococci and staphylococci on hospital fabrics and plastics. Journal of Clinical Microbiology, 38(2), pp. 724–726. DOI: https://doi.org/10.1128/JCM.38.2.724-726.2000
Otter, J.A., Yezli, S. and French, G.L. (2011). The role of contaminated surfaces in the transmission of nosocomial pathogens. Infection Control & Hospital Epidemiology, 32(7), pp. 687–699. https://doi.org/10.1086/660363 DOI: https://doi.org/10.1086/660363
Pal, S., Juyal, D., Adekhandi, S., Sharma, M., Prakash, R., Sharma, N. and Rana, A. (2015). Mobile phones: Reservoirs for the transmission of nosocomial pathogens. Advanced Biomedical Research, 4, p. 144. https://doi.org/10.4103/2277-9175.161553 DOI: https://doi.org/10.4103/2277-9175.161553
Rutala, W.A. and Weber, D.J. (2001) Surface disinfection: Should we do it? Journal of Hospital Infection, 48(Suppl A), pp. S64–S68. https://doi.org/10.1053/jhin.2001.0884 DOI: https://doi.org/10.1016/S0195-6701(01)90017-9
Smith, S.J., Young, V., Robertson, C. and Dancer, S.J. (2018). Where do hands go? An audit of sequential hand-touch events on a hospital ward. Journal of Hospital Infection, 100(1), pp. 75–81.
Ulger, F., Dilek, A., Esen, S., Sunbul, M., Leblebicioglu, H., and Saniç, A. (2009). Are healthcare workers’ mobile phones a potential source of nosocomial infections? Infection Control & Hospital Epidemiology, 30(3), pp. 290–292.
Wilson, J.A., Loveday, H.P., Hoffman, P.N. and Pratt, R.J. (2020). The role of environmental contamination in the transmission of healthcare-associated infections. Journal of Infection Prevention, 21(3), pp. 105–112.
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- 2025-12-16
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- Vol. 5 No. 2 (2025)
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- Original Articles
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