Prevalence and Antimicrobial Resistance Patterns of Uropathogenic Bacteria Isolated at Tobruk Medical Center, Tobruk, Libya: A 4-Year Retrospective Study

• Osamah Miftah Ahmed

  Department of Medical Laboratory, University of Tobruk, Tobruk, Libya.

  Author

• Miftah Larbid

  Department of Nutrition, University of Tobruk, Tobruk, Libya.

  Author

• Alkadafe Agelah

  Center for Research, Consulting, and Training, University of Tobruk, Tobruk, Libya.

  Author

The UTI is one of the most common bacterial diseases, and the emergence of antimicrobial resistance is now an acute risk to population health. The current study investigated the prevalence of Uropathogenic bacteria and their resistance trend in a sample of patients at the Tobruk Medical Centre. Methods: Between January 2021 and December 2024, 6,032 urine specimens were taken from patients suspected of UTI. Isolates were identified with standard microbiological and biochemical methods. Susceptibility was tested by Kirby-Bauer disk diffusion following CLSI (Clinical Laboratory Standards Institute) guidelines (20). Results: Significant bacterial growth was recovered from 1,264 specimens (20.95%). Gram-negative organisms dominated, with Escherichia coli as the leading isolate (49.36%), followed by Klebsiella spp. (16.85%) and Proteus spp. (11.07%). Staphylococcus aureus (14.79%) was the chief gram-positive isolate. Several isolates remained highly susceptible to nitrofurantoin and ciprofloxacin, but resistance increased against Augmentin, ceftazidime, Clarithromycin, and gentamicin. Conclusion: Gram-negative bacteria and Escherichia coli, in this case, were the main causative agents of UTIs. These findings highlight the need to have continuous surveillance and prudent use of antibiotics in an effort to strengthen the control of UTI in eastern Libya.

Aballu, H. Y. (2026). Antibiotic Resistance Patterns in Clinical Bacterial Isolates from Gharyan City, Libya. AlQalam Journal of Medical and Applied Sciences, 120. https://doi.org/10.54361/ajmas.269120

Anam, B., Mustafa, M., & Anjum, I. (2024). Empiric therapy for community acquired urinary tract infection in an era of increasing antimicrobial resistance. IP International Journal of Medical Microbiology and Tropical Diseases, 9(4), 267–274. https://doi.org/10.18231/j.ijmmtd.2023.050

Bush, N. G., Diez-Santos, I., Abbott, L. R., & Maxwell, A. (2020). Quinolones: mechanism, lethality and their contributions to antibiotic resistance. Molecules, 25(23), 5662. https://doi.org/10.3390/molecules25235662

CDC. (2026, July 15). Retrieved July 15, 2026, from https://www.cdc.gov/antibiotic-use/hcp/core-elements/index.html#print.

Elmansori, A., & Ellafi, E. (2025). Wound Infiltration Using Bupivacaine Versus Bupivacaine with Ketamine for Postoperative Analgesia Improvement in Cesarean Section Operations. Derna Academy Journal for Applied Sciences, 3(1), 66-75.

Elzouki, E. M., Eljamay, S. M., & Alshilwi, Y. A. (2024). Isolation and Identification of Escherichia Coli in Uropathogenesis. Derna Academy Journal for Applied Sciences, 2(2), 103-110.

Fakhralddin, S. S., Abdullah, P. A., Ibrahim, D. N., & Maarouf, K. B. (2026). Incidence and pattern of antimicrobial resistance of main bacterial isolates from patients with communityacquired urinary tract infections in North of Iraq. Archivio Italiano Di Urologia E Andrologia, 98(1), 14715. https://doi.org/10.4081/aiua.2026.14715

Fateh, R., & Ali, Q. (2025). Therapeutic Advances against Uropathogenic E. coli: From Beta-Lactamase Inhibition to Anti-Biofilm Agents. In Infectious diseases. https://doi.org/10.5772/intechopen.1010998

George, N., World Health Organization, & Paterson, D. L. (2021). ANTIMICROBIAL SUSCEPTIBILITY TESTING clinical and laboratory perspectives. PIONEERING DIAGNOSTICS.

Halawa, E. M., Fadel, M., Al-Rabia, M. W., Behairy, A., Nouh, N. A., Abdo, M., Abdeen, A. (2024). Antibiotic action and resistance: updated review of mechanisms, spread, influencing factors, and alternative approaches for combating resistance. Frontiers in Pharmacology, 14, 1305294.https://doi.org/10.3389/fphar.2023.1305294

Hamadalneel, Y., Ahmed, H., Alamin, M., Almahy, W., Almustafa, Z., Yousif, Y., & Taha, M. (2024). Prevalence and antimicrobial sensitivity patterns of Uropathogens in Wad Medani, Sudan: Three years, Cross-Sectional study. Infection and Drug Resistance, Volume 17, 2131–2140. https://doi.org/10.2147/idr.s464501

Harrell, J. E., Hahn, M. M., D’Souza, S. J., Vasicek, E. M., Sandala, J. L., Gunn, J. S., & McLachlan, J. B. (2021). Salmonella biofilm formation, chronic infection, and immunity within the intestine and hepatobiliary tract. Frontiers in Cellular and Infection Microbiology, 10, 624622. https://doi.org/10.3389/fcimb.2020.624622

Hasan, S. M., & Ibrahim, K. S. (2022). Molecular Characterization of Extended Spectrum β-Lactamase (ESBL) and Virulence Gene-Factors in Uropathogenic Escherichia coli (UPEC) in Children in Duhok City, Kurdistan Region, Iraq. Antibiotics, 11(9), 1246. https://doi.org/10.3390/antibiotics11091246

Hussain, H. I., Aqib, A. I., Seleem, M. N., Shabbir, M. A., Hao, H., Iqbal, Z., . . . Li, K. (2021). Genetic basis of molecular mechanisms in β-lactam resistant gram-negative bacteria. Microbial Pathogenesis, 158, 105040. https://doi.org/10.1016/j.micpath.2021.105040

Ibrahim, A. I., Abdelgyoum, H. A., Elfaki, N. S., Elbadawi, M. H., & Kashif, E. (2025). Bacterial etiology of urinary tract infections and their sensitivity patterns towards commonly used antibiotics in port Sudan City, Sudan: a retrospective study. BMC Infectious Diseases, 25(1), 1017. https://doi.org/10.1186/s12879-025-11437-w

Kala, K., Chakraverti, T. K., Kumar, S., & Kumar, A. (2026). Bacterial profile and antibiotic resistance patterns in urinary tract infections at a tertiary care hospital. International Journal of Current Pharmaceutical Review and Research, 18(01). https://doi.org/10.25258/ijcpr.18.1.171

Lee, A. C., Mullany, L. C., Koffi, A. K., Rafiqullah, I., Khanam, R., Folger, L. V., . . . Baqui, A. H. (2019). Urinary tract infections in pregnancy in a rural population of Bangladesh: population-based prevalence, risk factors, etiology, and antibiotic resistance. BMC Pregnancy and Childbirth, 20(1), 1. https://doi.org/10.1186/s12884-019-2665-0.

Lynch, J. P., Clark, N. M., & Zhanel, G. G. (2021). Escalating antimicrobial resistance among Enterobacteriaceae: focus on carbapenemases. Expert Opinion on Pharmacotherapy, 22(11), 1455–1474. https://doi.org/10.1080/14656566.2021.1904891

Murray, B. O., Flores, C., Williams, C., Flusberg, D. A., Marr, E. E., Kwiatkowska, K. M., Rohn, J. L. (2021). Recurrent urinary tract infection: a mystery in search of better model systems. Frontiers in Cellular and Infection Microbiology, 11, 691210. https://doi.org/10.3389/fcimb.2021.691210

Rojas, L., Ceron, I., Araos-Baeriswyl, E., Olivares, R., Valenzuela, A., & Aizman, A. (2024). Patrones de susceptibilidad antimicrobiana en pacientes adultos hospitalizados por infección del tracto urinario adquirida en la comunidad en dos hospitales terciarios de Santiago, Chile. Revista Médica De Chile, 152(3), 302–313. https://doi.org/10.4067/s0034-98872024000300302.

Shihab, A. M., & Jadallah, S. O. (2026). Antibiotic Resistance Among Gram-Negative Bacteria Isolated from Urinary Tract Infections. Derna Academy Journal for Applied Sciences, 6(1), 38-48.

Temesgen, A. B., & Shiferaw, S. A. (2025). Antimicrobial Multidrug Resistance and Mechanisms of Action: An Overview. BioMed Research International, 2025(1), 8847267. https://doi.org/10.1155/bmri/8847267Ullah, Z., Asghar,

J., Aziz, N., Ullah, A., Ashour, A. A., Felemban, M. F., Quddoos, A. (2025). Bacterial profiling and antibiotic resistance patterns in urinary tract infections: a microbiological analysis from Dera Ismail Khan, Pakistan. BMC Infectious Diseases, 25(1), 1151. https://doi.org/10.1186/s12879-025-11588-w

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