Clinical, Microbiological, and Hematological Assessment of Vibrio Infection in Flathead Grey Mullet (Mugil cephalus) Near the Port Benghazi, Libya

• Nagi Mousa

  General Trend Section, College Natural Resources and Environmental Sciences-Quba branch, Derna university, Libya.

  Author

• Ghaidan Khalil

  General Trend Section, College Natural Resources and Environmental Sciences-Quba branch, Derna university, Libya.

  Author

• Salama I Ahmadi

  Department Animal Production, Faculty of Agriculture, University of Omer Al-Mokhtar, Libya

  Author

• Yaser hamad

  Department Internal Medicine, Faculty of Veterinary Medicine, University of Omer Al-Mokhtar, Libya

  Author

The present study aimed to address the impact of Vibrio bacteria on the health status of Mugil Cephalus inhabiting the coastal area of Benghazi port. A total of 20 samples of (M. Cephalus) were collected at random from the Benghazi Harbour throughout the period from March 2025 to Augest 2025, All the specimen from the fish were examined outwardly and inwardly and lesions were registered. Bacterial isolation from the liver and kidney was done using the appropriate culture media. The infection rate was 30% . The external lesions during the spring and summer seasons were hemorrhages at the base of the pectoral fin and around the operculum, congestion of caudal and anal fins. While internal lesions were splenomegaly, congested liver, kidney, and swim bladder. Bacteriological identification was confirmed based on the cultural characteristics and biochemical activities of isolates, On adjusted TCBS medium the recovered colonies showed as circular yellow or green colonies ranging characteristic of Vibrio spp. , In regards to the hematological parameters associated with diseased and apparently healthy Mugil Cephalus. The total protein, and globulin were lower in diseased than apparently healthy Mugil Cephalus fish, Data were statistically analyzed using T-test to compare healthy and infected fish, and differences were considered significant at (p<0.05).

1. Ghaidan Khalil , General Trend Section, College Natural Resources and Environmental Sciences-Quba branch, Derna university, Libya.

    General Trend Section, College Natural Resources and Environmental Sciences-Quba branch, Derna university, Libya. 

Abdelaziz, M., Ibrahem, M. D., Ibrahim, M. A., Abu-Elala, N. M., & Abdel-moneam, D. A. (2017). Monitoring of different vibrio species affecting marine fishes in Lake Qarun and Gulf of Suez: Phenotypic and molecular characterization. The Egyptian Journal of Aquatic Research, 2(43), 141-146. https://www.sciencedirect.com/science/article/

Abdel-Tawwab, M., Monier, M. N., Hoseinifar, S. H., & Faggio, C. (2019). Fish response to hypoxia stress: growth, physiological, and immunological biomarkers. Fish physiology and biochemistry, 45(3), 997-1013.‏ https://link.springer.com/article

ABMDAS, M., & DEMİREL, E. (2018). A PRELIMANARY STUDY ON DEVELOPMENT PORT OF BENGHAZI. International Journal of Social and Humanities Sciences Research (JSHSR), 5(19), 615-626. https://jshsr.org/index.php/pub/article/view/1799

Austin, B. (2011). Taxonomy of bacterial fish pathogens. Veterinary research, 42, 1-13. https://link.springer.com/article/

Austin, B. (2019). Methods for the diagnosis of bacterial fish diseases. Marine Life Science & Technology, 1, 41-49. https://link.springer.com/article/

Austin, B., Austin, D. A., Austin, B., & Austin, D. A. (2016). Pseudomonads. Bacterial fish pathogens: disease of farmed and wild fish, 475-498. https://link.springer.com/chapter/

Balami, S., Sharma, A., & Karn, R. (2019). Significance of nutritional value of fish for human health. Malaysian Journal of Halal Research, 2(2), 32-34. https://sciendo.com/2/v2/download/article/10.2478/mjhr-2019-0012.pdf

bioMérieux. (2022). API 20E identification system: User's manual. bioMérieux. https://link.springer.com/content/pdf/

Coles, E.H. (1986). Veterinary Clinical Pathology 4th Edition. W.B. Saunders Co. Philadelphia. https://www.cabidigitallibrary.org/doi/full/10.5555/19672204505

De Souza Valente, C., & Wan, A. H. (2021). Vibrio and major commercially important vibriosis diseases in decapod crustaceans. Journal of Invertebrate Pathology, 181, 107527. https://www.sciencedirect.com/science/article/pii/

Doumas, B.T., Bayse, D.D., Carter, R.J., Peters, T., Jr., Schaffer, R. (1981). A candidate reference method for determination of total protein in serum. I. Development and validation. Clinical chemistry, 27, 1642-1650. https://academic.oup.com/clinchem/article-abstract

Elgendy, M. Y., Soliman, W. S., Hassan, H. A., Kenawy, A. M., & Liala, A. M. (2015). Effect of abrupt environmental deterioration on the eruption of vibriosis in mari-cultured shrimp, Penaeus indicus, in Egypt. Journal of Fisheries and Aquatic Science, 10(3), 146. https://www.academia.edu/download

Ellis, A.E. (1999). Immunity to bacteria in fish. Fish & shellfish immunology, 9, 291-308. https://www.sciencedirect.com/science/article

El-Sharaby, S., Abd-Elgaber, M., Tarabees, R., Khalil, R., Ali, M., & El-Ballal, S. (2018). Bacteriological and histopathological studies on Vibrio species isolated from naturally infected freshwater fish in Delta Region, Egypt. Adv. Anim. Vet. Sci, 6(1), 17-26. http://nexusacademicpublishers.com/uploads/files/AAVS_6_1_17-26.pdf

FAO. (2020). The State of Mediterranean and Black Sea Fisheries 2020. Food and Agriculture Organization of the United Nations. https://scholar.google.com/scholar

Sun, Y., Yan, Y., Yan, S., Li, F., Li, Y., Yan, L., ... & Bai, Y. (2024). Prevalence, antibiotic susceptibility, and genomic analysis of Vibrio alginolyticus isolated from seafood and freshwater products in China. Frontiers in Microbiology, 15, 1381457.‏ https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1381457/full

Gabriel, U.U., Ezeri, G.N.O., & Opabunmi, O.O. (2004). Influence of sex, source, health status and acclimation on the hematology of Clarias gariepinus (Burchell, 1822). African Journal of Biotechnology, 3, 463-467. https://www.ajol.info/index.php/ajb/article/view/14998

Helmi, A. M., Mukti, A. T., Soegianto, A., & Effendi, M. H. (2020). A review of vibriosis in fisheries: public health importance. Sys Rev Pharm, 11(8), 51-58. https://repository.unair.ac.id/124340/

Hrubec, T. C., Cardinale, J. L., & Smith, S. A. (2000). Hematology and plasma chemistry reference intervals for cultured tilapia (Oreochromis hybrid). Veterinary clinical pathology, 29(1), 7-12. https://onlinelibrary.wiley.com/doi/abs/

Harikrishnan, R., Rani, M. N., & Balasundaram, C. (2003). Hematological and biochemical parameters in common carp, Cyprinus carpio, following herbal treatment for Aeromonas hydrophila infection. Aquaculture, 221(1-4), 41-50.‏ https://www.sciencedirect.com/science/article/p

Kılıç, Z. (2021). Water pollution: causes, negative effects and prevention methods. İstanbul Sabahattin Zaim Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 3(2), 129-132. https://dergipark.org.tr/tr/pub/izufbed/issue/65793/100904

Kumar, S., Sahu, N.P., Pal, A.K., Choudhury, D., Yengkokpam, S., & Mukherjee, S.C. (2005). Effect of dietary carbohydrate on haematology, respiratory burst activity and histological changes in L. rohita juveniles. Fish & shellfish immunology, 19, 331-344. https://www.sciencedirect.com/science/article/pii

Lied, E., Gjerde, J., & Braekkan, O.R. (1975). Simple and Rapid Technique for Repeated Blood Sampling in Rainbow Trout (Salmo gairdneri). Journal of the Fisheries Research Board of Canada, 32, 699-701. https://cdnsciencepub.com/doi/abs/

Mohamad, N., Mustafa, M., Amal, M. N. A., Saad, M. Z., Md Yasin, I. S., & Al‐saari, N. (2019). Environmental factors associated with the presence of Vibrionaceae in tropical cage‐cultured marine fishes. Journal of Aquatic Animal Health, 31(2), 154-167. https://academic.oup.com/jaah/article-abstract/

OSHEBI, H. E., & El-EHFIFI, S. S. (2017). Assessment of chemical and biological pollution. https://www.researchgate.net/profile/Osama-Shaltami/publication/

Roberts, R. J. (2012). Fish pathology. John Wiley & Sons. https://books.google.com/books

Roux, F. L., Wegner, K. M., Baker-Austin, C., Vezzulli, L., Osorio, C. R., Amaro, C., ... & Blokesch, M. (2015). The emergence of Vibrio pathogens in Europe: ecology, evolution, and pathogenesis (Paris, 11–12th March 2015). Frontiers in microbiology, 6, 830. https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2015.00830/full

Shaltami, O. R., Fares, F. F., El-Fallah, O. A., & EL-FETORI, H. M. (2019). Parasitic infections in some fish species from the Libyan coast of the Mediterranean Sea. https://www.researchgate.net/publication/

Shinn, A. P., Pratoomyot, J., Bron, J. E., Paladini, G., Brooker, E. E., & Brooker, A. J. (2015). Economic costs of protistan and metazoan parasites to global mariculture. Parasitology, 142(1), 196-270. https://www.cambridge.org/core/journals/parasitology/article/

Southgate, P. (1985). Investigation into mortalities of grouper spp. off Libyan coast. FAO: Final report of a mission. https://www.google.com/search

Whitfield, A. K., Panfili, J., & Durand, J. D. (2012). A global review of the cosmopolitan flathead mullet Mugil cephalus Linnaeus 1758 (Teleostei: Mugilidae), with emphasis on the biology, genetics, ecology and fisheries aspects of this apparent species complex. Reviews in Fish Biology and Fisheries, 22(3), 641-681. https://link.springer.com/article/

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